![]() ![]() We used simulated data representing a wide range of population sizes, sample sizes and number of loci. We evaluated the performance of two Ne estimators to detect population declines: the two-sample temporal method and a one-sample method based on linkage disequilibrium (LD). The studied populations, located at the rear edge of the species European distribution, reveal a wealth of genetic variation that is very valuable for the genetic conservation of local adaptive gene complexes, especially under contemporary climatic change scenarios.Įarly detection of population declines is essential to prevent extinctions and to ensure sustainable harvest. avium populations studied, genetic stochasticity events were not apparent. In general, it appears that despite the particular characteristics of the P. A possible bottleneck was detected for only one population. According to linkage disequilibrium analysis, estimates of effective population size were generally sufficient for maintaining extant genetic variability and evolutionary potential. AMOVA also detected significant differentiation among populations for ISSRs (Φ ST = 0.338) and SRRs (Φ ST = 0.162). We observed moderate population differentiation for ISSR (G ST = 0.113) and SSR (F ST = 0.097) markers. Populations presented a relatively high level of genetic variation, with a mean genetic diversity of H e = 0.166 and H e = 0.740 regarding ISSR and SSR analysis, respectively. ![]() Five populations from northern Greece were evaluated based on 46 ISSR and 11 SSR loci. In this study, we used dominant inter simple sequence repeat (ISSR) and codominant simple sequence repeat (SSR) markers to infer the genetic structure of P. avium populations prone to potential stochastic, genetic, and demographic events. These characteristics, especially at the southern limits of the species natural distribution where additional varying stresses may occur, render P. Wild cherry (Prunus avium L.) is a widespread, partially asexual, noble hardwood European species characterized by a scattered distribution, small population sizes, and human exploitation for its valuable wood. ![]()
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